Antioxidant Activities in Germinating Mungbean

Authors

  • Irvia Resti Puyanda Universitas Slamet Riyadi
  • Uyun Nurul Aini Khon Kaen University

Keywords:

mungbean, germination, moisture content, antioxidant activity

Abstract

The objectives of this work were to investigate the influence of  germination times (24,48, 72, 96, 120 h) and conditions (light and dark) on the moisture content and antioxidant activity in mungbean sprout. The antioxidant activity were analyzed using in vitro methods. ABTS and DPPH methods were used to measure the antioxidant activity. A significant increase (p-value < 0.05) was observed under ABTS method at 72 and 96 h, while it decreased at longer germination times. In addition, using DPPH method decreased the antioxidant activity significantly (p-value < 0.05) from 24 to 48 h, however, it increases again at 72 and 96 h. Germination times and conditions significantly affects (p-value < 0.05) the antioxidant activities of mungbean sprout. Based on the results, germination under dark conditions enhanced lower antioxidant activities during germination process.

References

AOAC. 2012. Official Methods Of Analysis Of The Association Of Official Analytical Chemist. Association of Official Analytical Chemist. Washington , D.C.

Charles, D.J. 2013. Antioxidant Properties of Spices, Herb, and Other Sources. Springer. New York.

Chen, Z., Yu, L., Wang, X., Gu, Z., Beta, T. 2016. Changes of phenolic profiles and antioxidant activity in canary seed (Phalaris canariensis L.) during germination. Journal of Food Chemistry 194:608-618. DOI: 10.1016/j.foodchem.2015.08.060.

Damiani, E., Astolfi, P., Carloni, P., Stipa, P., Greci, L. 2008. Antioxidants: How they work. Oxidants in Biology: A Question of Balance, pp. 251-266.

Djamil, R. Anelia, T. 2009. Phytochemical screening, bslt test, and antioxidant activity test of methanol extract of several species of Papilionaceae. Jurnal Ilmu Kefarmasian Indonesia 7(2): 65-71. (In Bahasa Indonesia)

Falcinelli B., Marconi O., Maranghi S., Lutts, S., Rosati, A., Famiani, F., Benincasa, P. 2017. Effect of genotype on the sprouting of pomegranate (Punica granatum L.) seeds as a source of phenolic compounds from juice industry by-products. Journal of Plant Foods for Human Nutrition 72(4):432-438. DOI: 10.1007/s11130-017-0645-y.

Guo, X., Li, T., Tang, K., Liu, R.H. 2012. Effect of germination on phytochemical profiles and antioxidant activity of mung bean sprouts (Vigna radiata). Journal of Agricultural and Food Chemistry 60: 11050-11055. DOI: 10.1021/jf304443u.

Huang, D., Boxin, O., Prior, RL 2005. The chemistry behind antioxidant capacity assays. Journal of Agriculture Food Chemistry, 53: 1841-1856. DOI: 10.1021/jf030723c

Karakaya, S. 2004. Bioavailability of phenolic compounds. Critical Reviews in Food Science and Nutrition 44(6): 453–464. DOI: 10.1080/10408690490886683.

Khattak, A. B., Zeb, A., Bibi, N., Khalil, S. A., Khattak, M. S. 2007. Influence of germination techniques on phytic acid and polyphenols content of chickpea (Cicer arietinum L.) sprouts. Food Chemistry Journal. 104: 1074-1079. DOI: 10.1016/j.foodchem.2007.01.022.

Leong, L.P., Shui, G. 2002. An investigation of antioxidant capacity of fruits in Singapore markets. Food Chemistry 76(1): 69-75. DOI: 10.1016/S0308-8146(01)00251-5.

Lin, P.Y., Lai, H.M. 2006. Bioactive compounds in legumes and their germinated products. Journal of Agricultural and Food Chemistry 54(11), 3807-3814. DOI: 10.1021/jf060002o.

Liu, H., Chen, Y., Hu, T., Zhang, S., Zhang, Y., Zhao, T., Yu, H., Kang, Y. 2016. The influence of light-emitting diodes on the phenolic compounds and antioxidant activities in pea sprouts. Journal of Functional Foods 25: 459–465. DOI: 10.1016/j.jff.2016.06.028.

Mayerand, A.M., Shain, Y. 1974. Control of seed germination. Annual Review Plant Physiology 25: 167-193. DOI: 10.1146/annurev.pp.25.060174.001123.

Naczk, M., Shahidi, F. 2004. Extraction and analysis of phenolics in food. Journal of Chromatography A 1054(1-2): 95–111. DOI: 10.1016/j.chroma.2004.08.059.

Puyanda, I.R 2015. Effects of pH and temperature on activity and stability of glucoamylase in mung bean (Vigna radiata) variety Vima-1. Bachelor Thesis. Universitas Gadjah Mada, Yogyakarta. ( In Bahasa Indonesia)

Scalbert A., Manach C., Morand C., Remesy C., Jimenez L. 2005. Dietary polyphenols and the prevention of diseases. Critical Review Food Science Nutrition 45(4): 287-306. DOI: 10.1080/1040869059096.

Shahidi, F. and Ambigaipalan, P. 2015. Phenolics and polyphenolics in foods, beverages, and spices: Antioxidant activity and health effects – A review. Journal of Functional Food 18: 820-897. DOI: 10.1016/j.jff.2015.06.018.

Stratil P, Klejdus B, Kuban V. 2006. Determination of total content of phenolic compounds and their antioxidant activity in vegetables evaluation of spectrophotometric methods. Journal Agriculture and Food Chemistry 54(3): 607-616. DOI: 10.1021/jf052334j.

Vale, A.P., Cidade, H., Pinto, M., Oliveira, M.B.P. 2014. Effect of sprouting and light cycle on antioxidant activity of Brassica oleracea varieties. Food Chemistry 165: 379-387. DOI: 10.1016/j.foodchem.2014.05.122.

Wisaniyasa, N. W. and Darmayanti, L. P. T. 2019. Kajiantotal fenol, flavonoid dan aktivitas antioksidan kacang merah (Phaseolus vulgaris L.) pada berbagai lama waktu perkecambahan. Scientific Journal of Food technology, 6(1)

Winarsi H. 2007. Natural Antioxidants and Free Radicals. Kanisius: Yogyakarta. (In Bahasa Indonesia)

Yuan, G., Wang, X., Guo, R., Wang, Q. 2010. Effect of salt stress on phenolic compounds, glucosinolates, myrosinase, and antioxidant activity in radish sprouts 121(4):1014-1019. DOI: 10.1016/j.foodchem.2010.01.040

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Published

2023-06-20

How to Cite

Puyanda, I. R., & Aini, U. N. (2023). Antioxidant Activities in Germinating Mungbean . Jurnal BETAHPA, 2(1 Juni), 19–25. Retrieved from https://ejournal.pppmitpa.or.id/index.php/BETAHPA/article/view/131